Fig. 9.

Binding (means ± S.D.) of immunoreactive ficolin to PRRSV-coated wells, detected by ELISA with polyclonal antibody to ficolin. Significant (p < 0.0001) GlcNAc-competible binding above wells containing 100 mM GlcNAc pre-incubation (open bars) was observed for pig plasma (PP), GlcNAc-affinity-purified plasma ficolin (pFCN), and recombinant porcine ficolin α (rFCN). There was insignificant GlcNAc-competible binding of immunoreactive ficolin observed in ficolin-depleted plasma (FDP), in lysate from Marc-145 cells without PRRSV (control 1) and in lysates from Lac Z-transfected CHO K1 cells without FCN (Control 2). Bars are from three separate experiments, each performed in triplicate.