Supplementary Figure 1.

The 3 in vitro HBV models used: stably transfected HepG2215 cells, transiently transfected HuH-7 cells, and HBV-infected HepaRG cells, with the time points of sample collection after treatment with cyclophilin inhibitors: alisporivir (ALV), or NIM (NIM811). During treatment with the compounds used (ALV, NIM, or telbivudine) for all cell lines, the culture medium including fresh compound was replaced every 24 hours. Cells and supernatants were collected at each time point as shown. HepaRG cells were infected with HBV derived from 5-day culture supernatant of AD38 cells, in the presence of 4% PEG8000. After 16 hours, the cells were washed thoroughly and the culture medium replaced with fresh William’s Medium E with HepaRG Maintenance Medium Supplement plus GlutaMAX, containing different concentrations of the test compounds ALV or NIM811. HepaRG cell viability, before adding the compounds and after 7-day culture, was checked with trypan blue exclusion and always exceeded 90%.