Supplementary Figure 3.

NIM811 also suppresses HBV replication and HBsAg secretion in HepG2215 cells and HepaRG. HepG2215 and HepaRG cells were treated with 0.25, 1, and 5 μg/mL of NIM811 and ALV over 72 hours and HBV DNA was quantitated by real-time PCR. Cells and supernatants were collected every 24 hours. HepG2215 cells: (A and B) secreted-virus (SV) HBV DNA and (C and D) intracellular nucleocapsid-associated (INA) HBV DNA. HepaRG: (E and F) INA HBV DNA. Mean values and the SD of 3 independent experiments are shown. *P values less than .05, **P values less than .01, and ***P values less than .001 for NIM811-treated vs nontreated cells. The ALV-treated figures were added for comparison purposes.