Supplementary Figure 6.

Dose-dependent inhibition of cellular cyclophilin expression by cyclophilin-specific siRNA. Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by GAPDH-specific siRNA and transfection efficiency (siGLO). HepG2215 cells were transfected with CypA, B, C, and D siRNA at 30, 60, 100, and 150 nmol/L for 24, 48, 72, and 96 hours. (A) CypA, (B) CypC, and (C) CypD expressions were measured in the cyclophilin siRNA-treated cells by real-time PCR. As controls. HepG2215 cells were transfected with GAPDH siRNA and siGLO transfection indicator for 48 hours. (D) GAPDH expression was measured in GAPDH and CypA siRNA-treated cells at BL (48 h after transfection), and at 24, 48, and 72 hours by real-time PCR. (E) Transfection efficiency was measured by flow cytometry at BL and 72 hours. ND, not done. The mean values and the SD of 2 independent experiments are shown. FITC, fluorescein isothiocyanate; SSC, side scatter.