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. 2014 Oct 8;148(2):403–414.e7. doi: 10.1053/j.gastro.2014.10.004

Figure 3.

Figure 3

Silencing of CYPA suppresses HBV replication and HBsAg secretion. HepG2215 cells were transfected with CYPA siRNA and seeded in a culture plate for 48 hours and then treated with 0.25, 1, and 5 μg/mL of ALV over 72 hours. Cells and supernatants were collected every 24 hours. (A) Secreted-virus (SV) HBV DNA, (B) intracellular nucleocapsid-associated (INA) HBV were quantified by real time PCR. (C) sHBsAg was measured by ELISA. Mean values and the SD of 3 independent experiments are shown. *P values less than .05, **P values less than .01, and ***P values less than .001 for CYPA siRNA ± ALV-treated vs scrambled siRNA-treated cells.