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. 2014 Oct 8;148(2):403–414.e7. doi: 10.1053/j.gastro.2014.10.004

Figure 5.

Figure 5

siRNA silencing of CYPC and CYPD decreases HBV replication and HBsAg secretion but to a lesser extent than CYPA. HepG2215 cells were transfected with CYPC and CYPD siRNA and seeded in a culture plate for 48 hours and then treated with 0.25, 1, and 5 μg/mL of ALV over 72 hours. Cells and supernatants were collected every 24 hours. (A and D) Secreted-virus (SV) HBV DNA and (B and E) intracellular nucleocapsid-associated (INA) HBV DNA were quantified by real-time PCR. (C and F) SHBsAg was measured by ELISA. Mean values and the SD of 3 independent experiments are shown. *P values less than .05, **P values less than .01, ***P values less than .001 for CYPC/D siRNA ± ALV-treated vs scramble siRNA-treated cells. †P values less than .05, ††P values less than .01, †††P values less than .001 for CYPC/D siRNA-treated vs CYPC/D siRNA+ALV-treated cells.