Table 1.

Oligonucleotides used in this study.

Oligonucleotide	Sequence (5′–3′)	Use and properties
iPCR F	CACAAAGCCGGCGCTTACAGACAAGCTGTG	Construction of pJAD1 and pJAD2; T7Te terminator sequences are underlined, StuI restriction sites are indicated in italics and PmeI restriction sites are indicated in bold
iPCR R	GAAACAGCCGGCCTCAAAGGCGGTAATACG’
Cas1F	AGGCTCACCTTCGGGTGGGCCTTTCTGCGATATaggcctGGGAATTTAATACGACTCACTATAGTT
Cas1R	AgtttaaacaGGCTCACCTTCGGGTGGGCCTTTCTGCGATATaggcctCAAAAAACCCCTCAAGACCCGTTTAG
Cas2F	AgtttaaacaAGGCTCACCTTCGGGTGGGCCTTTCTGCGATATGGGAATTTAATACGACT
Cas2R	CGAAGgtttaaacGGCTCACCTTCGGGTGGGCCTTTCTGCGCAAAAAACCCCTCAAGACCCGTTTAG
InvF	GGCCGGCATGGTCCCAGC	Linearization of pJAD1 and pJAD2 for cloning
InvR	TATAGTGAGTCGTATTA
S1F	GTTATTTGAGCGATGGTCATCACCG	AHSV-4 genome segment-specific primers
S1R	GTAAGTGTTTTGAGCTGTGGGG
S2F	GTTAATTCACTATGGCGTCCGAG
S2R	GTATGTGTATTCACATGGAGCAAC
S3F	GTTTATTTTCTGCTGTGAACTCCAG
S3R	GTAAGTGTAATTCTGCCCGC
S4F	GTTATTTAGGATGGAACCTTACG
S4R	GTAAGGTTATTAGGATTCCCC
S5F	GTTAAAGAACCTAGGCGGTTTGG
S5R	GTAAGTTTGTGAACCAGGGGG
S6F	GTTATTTTTCCAGAAGCCATGG
S6R	GTAAGTGTTTTTCCCGCCCAC
S7F	GTTAAATTCGGTTAGGATGGACG
S7R	GTAAGTGTATTCGGTATTGACGTATTAC
S8F	GTTAAAAATCCGTTCGTCATCATGG
S8R	GTATGTTGAAATCCGCGGTTAC
S9F	GTTAAATAAGTTGTCTCATGTCTTCGGC
S9R	GTAAGTTTTAAGTTGCCTGCAAG
S10F	GTTAATTATCCCTTGTCATGAATCTAGC
S10R	GTAAGTTGTTATCCCACCCCCTAG
S5PstI-F	GGGACATCTGCAGCCGGAATCAATTTTAGT	AHSV-4 S5 mutagenic primers
S5PstI-R	ACTAAAATTGATTCCGGCTGCAGATGTCCC
2.1F	GTTAATTCACTATGGCGTCCGAG	AHSV-1 genome segment-specific primers
2.1R	GTAAGTGTTTTGAGCTGTGGGG
6.1F	GTTTATTTTTCCAGAAGCCATGG
6.1R	GTAAGTGTTTTTCCCGCCCAC
T7Bam-F	GAggatccATGAACACGATTAACATCGCTAAGAACGACTTC	Amplification of T7 RNA polymerase coding region from E. coli BL21(DE3) cells; BamHI restriction sites indicated in lower case letters
T7Bam-R	GCggatccTTACGCGAACGCGAAGTCCGACTCTAAGATGTC
CMV-F	CCgatatcGAGGCGGAAAGAACCAGCTGGGGCTCTA	Amplification of expression cassette from pcDNA-T7pol; EcoRV restriction sites indicated in lower case letters
CMF-R	CCgatatcGTTGACATTGATTATTGACTAGTTA