Table 1.

Data collection and phasing statistics for the head-binding domain

Parameters	Native	TEMEa	UOACb	UOTEc
Total observations	48,766	64,266	33,849	35,960
Unique reflections	20,822	28,937	18,290	18,811
Resolution (Å)	2.6	2.3	2.7	2.7
Completeness (%)d	96.5/72.2	95.4/82.9	94.7/84.0	97.4/86.5
Rmerge (%)e	7.6/29.8	5.6/17.4	8.2/32.1	9.8/28.4
Riso (25–3.0 Å)f	—	0.21	0.21	0.25
Heavy atom sites	—	6	9	7
RCullisg	—	0.62/0.69	0.93/0.92	0.65/0.71
Phasing powerh	—	2.16/1.41	2.0/1.29	0.61/0.45

^aMet(Te)-crystal.

^b15 mM of UO₂-acetate, one day.

^c15 mM of UO₂-acetate in Met(Te)-crystal, one day.

^d25–2.60/2.60–2.65 Å for Native; 25–2.3/2.30–2.35 Å for TEME; 25–2.8/2.8–2.9 Å for UOAC and UOTE.

^eR_merge =∑_h∑_i|I(h, i)| − |〈I(h)〉|/∑_h∑_iI(h, i), where I(h, i) is the intensity value of the ith measurement of h and 〈I(h)〉 is the corresponding mean value of h for all I measurements of h: the summation is over all measurements.

^fR_iso =∑|F_PH² − F|/∑(F_PH²F_P²), where F_PH and F_P are the derivative and the native structure factor amplitudes, respectively.

^gR_Cullis =∑_hkl|F_PH ± F_p| − F_H(calc.)|/∑_hkl|F_PH ± F_P|

^hF_H/residual; rms mean heavy-atom contribution/ rms residual, defined as [(F_PHC² − F_PH² −)²/n]^1/2 with the sum over all reflections, where FPHC is the calculated structure factor and FPH is the structure factor amplitude of the heavy-atom derivative, respectively.