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Analytical sensitivity of the conventional RT–PCR and SYBR Green real-time RT–PCR assays for ISAV in TO cells
| Detection format | Template dilution |
|||||||
| Neat | 1:101 | 1:102 | 1:103 | 1:104 | 1:105 | 1:106 | 1:107 | |
| Gel electrophoresis of conventional one-tube RT–PCR products | 3/3a | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| Real-time RT–PCR format | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 |
| Gel electrophoresis of real-time RT–PCR products | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Number of replicas positive out of total tested.
