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. 2004 Jan 22;117(1):37–47. doi: 10.1016/j.jviromet.2003.11.020

Table 4.

Determination of absolute viral RNA levels at various times post-ISAV inoculation in TO cells by extrapolation of Ct values from the standard curve

Post-inoculation (h) CPEa Mean Ct valueb, c ISAV RNA levels (μg/μl)
0 − 25.370 0.001
32 − 18.600 0.117
48 − 16.910 0.331
72 − 16.770 0.363
96 + 16.295 0.478
120 + 15.780 0.653
144 + 14.730 1.238
a

CPE denotes virus-induced cytopathic effects in cell culture.

b

Ct values are averages of duplicate samples except for samples harvested at 72 and 144h that represent Ct values of single samples.

c

The difference among mean Ct values of ISAV RNA levels in TO cells at different hours post-infection was significant (P≥0.05) at 5% α level.