Table 5.
Ct values of ISAV U5575-1 in CHSE-214 cells at various hours post-inoculationa
| Post-inoculation (h)b | Mean Ct valuec | Standard deviation |
| 0 | 20.105 | 0.757 |
| 32 | 21.005 | 0.856 |
| 48 | 21.000 | 0.042 |
| 72 | 21.200 | 0.000 |
| 96 | 21.485 | 1.407 |
| 120 | 20.565 | 0.035 |
| 144 | 20.805 | 0.163 |
The difference between the mean Ct values of ISAV in CHSE-214 cells at various hours post-inoculation was not statistically significant (P≤0.05).
Another CHSE-214 monolayer inoculated with ISAV U5575-1 strain was incubated until day 15 post-inoculation, to monitor for evidence of CPE.
Each mean Ct value is representative of two replicas.