Table 2.
Primers used for cDNA synthesis and full-length PCR of the coding region of the viral RNA segments L, M, and S. Primers were designed based on the genome of SBV isolate B80/11-4 (GenBank accession numbers HE649912, HE649913, HE649914 for L, M, and S segment, respectively), encompassing the termini and extending into the interior of the respective gene. The length of the primers were calculated by the G/C-A/T rule aiming at a melting temperature of 62–64 °C. Numbers in the primer names refer to the respective nt positions.
| SBV RNA segment | Primer orientation | Primer name | Primer sequence (5′ → 3′) |
|---|---|---|---|
| L | Forward | SBV_L1_F | CTAATTACAATCACTATGGAGAC |
| Reverse | SBV_L6864_R | GTGCCCCTAATTACATGAAACT | |
| M | Forward | SBV_M1_F | CCACAATCAAAATGCTTCTCAAC |
| Reverse | SBV_M4398_R | TATTGTCAGTCTCCAATAGAAAG | |
| S | Forward | SBV_S1_F | CTCCACTATTAACTACAGAAATAT |
| Reverse | SBV_S830_R | AGTAGTGTTCTCCACTTATTAAC |