Table 4.
Mutations identified through comparative nt sequencing of the three SBV isolates that were passaged 10 times in SK-6 cells. Complete L, M and S ORF sequences were determined from SBV before and after 10 consecutive passages. No mutations were found in the S ORF.
| SBV isolate no. | Genome segment | nt Change | aa Change | Type of aa change* (C/NC) |
|---|---|---|---|---|
| 96.1 | L | A6518C | Asn → Thr | C |
| M | C1813T | Thr → Ile | NC | |
| M | C3413T | Silent | na | |
| 100.3 | L | C202T | Silent | na |
| L | G4278A | Silent | na | |
| L | A4719G | Silent | na | |
| M | G1505A | Silent | na | |
| M | G1605A | Glu → Lys | NC | |
| 175.2 | M | T2051C | Silent | na |
| M | G2079A | Glu → Lys | NC |
*
C, conservative aa change; NC, non-conservative aa change; na, not applicable.