Table 1.
Detection by RT-PCR and ISH of Italy 02 virus in chickens infected at one day of age. Results are expressed as the number of positive birds per total of birds tested. In bold letters is represented the last positive result obtained. ND = Not done. NA = Not available.
| Tissues |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RT-PCR |
ISH |
||||||||||||
| Trachea | Nasal swab | Kidney | Cloacal swab | Nasal turbinate | Trachea | Lung | Kidney | Proventriculus | Duodenum | Caecal tonsil | Rectum | Bursa Fabricius | |
| 1 day PI | 3/3 | NA | 1/3 | 0/3 | 3/3 | 1/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| 3 days PI | 3/3 | NA | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | 3/3 | 2/3 |
| 5 days PI | 3/3 | 2/3 | 3/3 | 3/3 | 3/3 | 2/3 | 0/3 | 3/3 | 0/3 | 0/3 | 2/2 | 3/3 | 1/3 |
| 7 days PI | 3/3 | 2/3 | 3/3 | 2/3 | 3/3 | 3/3 | 0/3 | 1/3 | 0/3 | 0/3 | 3/3 | 3/3 | 1/3 |
| 9 days PI | 3/3 | 2/3 | 3/3 | 3/3 | 2/3 | 3/3 | 1/3 | 3/3 | 0/3 | 0/3 | 3/3 | 3/3 | 0/3 |
| 12 days PI | 3/3 | 1/3 | 2/3 | 3/3 | 1/3 | 1/3 | 0/3 | 2/3 | 0/3 | 0/3 | 0/3 | 2/3 | 0/1 |
| 15 days PI | 3/3 | 0/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 3/3 | 0/3 | 0/3 | 1/3 | 3/3 | 0/3 |
| 18 days PI | 2/3 | 0/3 | 0/3 | 2/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/2 | 2/3 | 0/3 |
| 21 days PI | 3/3 | 0/3 | 1/3 | 1/3 | ND | ND | ND | ND | 0/3 | 0/3 | 2/3 | 2/3 | 0/3 |
| 24 days PI | 0/3 | 0/3 | 0/3 | 3/3 | ND | ND | ND | ND | 0/3 | 0/3 | 2/2 | 3/3 | 0/3 |
| 27 days PI | 0/3 | 1/3 | 0/3 | 2/3 | ND | ND | ND | ND | 0/3 | 0/3 | 0/3 | 1/3 | 0/3 |