Table 2.
Detection of Italy 02 virus by RT-PCR and ISH in hens infected at 40 weeks of age. Results are expressed as the number of positive birds per total of birds tested. In bold letters the last positive result obtained. ND = Not done.
| Tissues |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RT-PCR |
ISH |
|||||||||||||
| Trachea | Nasal swab | Kidney | Cloacal swab | Nasal turbinate | Trachea | Lung | Kidney | Proventriculus | Duodenum | Caecal tonsil | Rectum | Ovary | Uterus | |
| 2 days PI | 3/3 | 1/3 | 2/3 | 2/3 | 3/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 2/2 | 2/2 | 0/3 | 0/3 |
| 4 days PI | 3/3 | 1/3 | 3/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 1/3 | 0/3 | 3/3 | 3/3 | 0/3 | 0/3 |
| 6 days PI | 3/3 | 1/3 | 2/3 | 3/3 | 3/3 | 3/3 | 0/3 | 0/3 | 1/3 | 0/3 | 3/3 | 3/3 | 0/3 | 0/3 |
| 8 days PI | 3/3 | 1/3 | 2/3 | 3/3 | 1/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | 2/3 | 0/3 | 0/3 |
| 11 days PI | 2/3 | 0/3 | 2/3 | 3/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 2/3 | 1/3 | 0/3 | 0/3 |
| 15 days PI | 1/3 | 0/3 | 2/3 | 3/3 | ND | ND | ND | ND | 0/3 | 0/3 | 2/2 | 3/3 | 0/3 | 0/3 |
| 19 days PI | 1/2 | 0/2 | 1/2 | 1/2 | ND | ND | ND | ND | 0/2 | 0/3 | 1/2 | 1/2 | 0/2 | 1/2 |