Fig. 2.

Detection of SV40 viral genome after capsid preparation using real-time PCR and DOP-PCR. Ten-fold serial dilutions of virus stock (measured in PFU) were mixed with 10⁶ Vero cells and examined both prior to and after capsid preparation. The SV40 viral nucleic acid was extracted from the viral capsids and 1/10 of the DNA was used as template for amplified using real-time and DOP-PCR in parallel. Detected quantities of viral nucleic acid are shown for each original sample. DOP-PCR results (+ or −) are shown at the top of the graph.