Table 1.

Design and optimization of degenerate oligonucleotide primer used in the universal viral nucleic acid amplification

Primer number	5′ specific sequence	Ambiguous sequence	3′-anchor sequence	Detection limit
1	CCGACTCGAG	NNNNNN	ATGTGG	106
2	CCGACTCGAG	INNNNNN	TGTGG	102
3	CCGACTCGAG	INNNNNN	TGTG	105
4	CCGACTCGAG	IINNNNNN	GTGG	>106
5	CCGACTCGAG	IIINNNNNN	GTGG	103
6	CCGACTCGAG	NNNNNNIII	GTGG	>106
7	CCGACTCGAG	IINNNIINNNI	TGTG	105
8	CCGACTCGAG	IINNNNNN	TGTG	103
9	CCGACTCGAG	IINNNIINNN	TGTG	>106
10	CCGACTCGAG	IINNNIINNN	TGT	>106
11	CCGACTCGAG	IIINNNNNN	TGG	104
12	CCGACTCGAG	IINNNIINNNI	TG	>106
13	GCGCCATCAG	INNNNNN	TGTGG	102
14	GCCCGCTCAG	INNNNNN	TGTGG	102
15	CCGACTCGAG	INNNNNN	TTCTG	102
16	CCGACTCGAG	INNNNNN	TCTG	105
17	CCGACTCGAG	INNNNNN	TTCT	105
18	CCGACTCGAG	INNNNNN	GTCT	105
19	CCGACTCGAG	IINNNNNN	TTCT	>106