Table 1.
Competitive interference within the FLUA/B/RSV assay.
| Single target |
FLU A H+ (Ct 17) |
FLU B H+ (Ct16) |
RSV-A H+ (Ct18) |
RSV-B H+ (Ct19) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution | FLUA (Ct) |
FLUB (Ct) |
RSVA (Ct) |
RSVB (Ct) |
IC# (Ct) |
FLUB (Ct) |
RSVA (Ct) |
RSVB (Ct) |
IC# (Ct) |
FLUA (Ct) | RSVA (Ct) | RSVB (Ct) | IC# (Ct) |
FLUA (Ct) | FLUB (Ct) | IC# (Ct) |
FLUA (Ct) | FL-B (Ct) | IC# (Ct) |
| 1 | 21.0 | 19.7 | 22.8 | 24.0 | <36^ | 19.2 | 23.4 | 23.1 | – | 20.9 | 23.9 | 23..4 | >39^ | 21.0 | 19.3 | – | 21.0 | 19.7 | >39^ |
| 2 | 23.9 | 22.0 | 26.3 | 27.4 | <36 | 21.7 | 27.0 | 28.6 | – | 23.3 | 27.1 | 28.0 | <36^ | 23.3 | 22.1 | – | 23.3 | 22.2 | >39^ |
| 3 | 24.7 | 22.9 | 27.6 | 28.3 | <36 | 22.8 | 28.4 | 30.4 | – | 24.2 | 27.9 | 28.9 | <36^ | 24.4 | 22.9 | – | 24.2 | 23.1 | >39^ |
| 4 | 27.3 | 25.5 | 30.4 | 31.0 | <36 | 25.4 | 32.6 | 34.3 | – | 26.7 | 30.1 | 31.8 | <36^ | 27.0 | 26.1 | >36 | 27.3 | 25.8 | <36 |
| 5 | 28.2 | 27.0 | 31.1 | 32.4 | <36 | 27.5 | 35.2 | 36.7 | – | 28.2 | 31.2 | 32.3 | <36^ | 28.1 | 26.6 | >36 | 28.4 | 27.1 | <36 |
| 6 | 30.0 | 29.4 | 33.0 | 33.2 | <36 | 32.8 | 36.3 | 40.6 | – | 30.2 | 33.8 | 35.3 | <36 | 30.2 | 29.2 | >36 | 31.1 | 29.7 | <36 |
| 7 | 31.7 | 30.2 | 34.8 | 35.3 | <36 | 34.9 | 38.2 | 40.7 | – | 31.1 | 35.1 | 36.1 | <36 | 31.5 | 30.5 | <36 | 32.1 | 30.3 | <36 |
| 8 | 34.6 | 31.5 | 37.1 | 37.2 | <36 | 39.8 | 40.6 | – | – | 34.2 | 36.8 | 40.0 | <36 | 36.4 | 35.1 | <36 | 35.0 | 34.0 | <36 |
| 9 | 36.1 | 33.5 | 37.9 | 37.7 | <36 | 40.8 | 44.1 | – | – | 36.1 | 39.6 | 39.7 | <36 | 36.6 | 34.1 | <36 | 35.6 | 33.0 | <36 |
| 10 | 36.6 | 35.4 | 41.5 | 40.4 | <36 | 41.8 | – | – | – | 36.0 | 39.1 | 42.3 | <36 | – | 39.2 | <36 | 40.4 | 36.0 | <36 |
#Average IC in single target samples = Ct 32.9 (32.1–34.6), IC Ct>40 was considered negative, ^IC FLU-A >40 or negative.