Table 2.

Cycle threshold (C_t) values for 27 patient samples containing seasonal influenza A virus and 22 patient samples containing A/H1N1v as assessed by the in-house RT-PCR version, the RealStar kit and reference assays for A/H1N1v³ (based on the hemagglutinin gene) and general influenza A³ (based on the matrix gene), respectively.

	Ct value
Sample	In-house RT-PCR	RealStar kit	Reference assay
Seasonal influenza A virus
1	29.4	27.3	29.1
2	29.7	25.9	28.5
3	34	27.7	31.5
4	26.8	23.3	25.7
5	23.8	19.3	21.7
6	30.6	25.9	29.6
7	27.6	24.1	26.2
8	31.6	26.6	30.2
9	33.9	30.0	32.6
10	37.9	>40	36.9
11	35.6	36.9	33.6
12	34.9	28.8	32.8
13	33.8	33.1	32.8
14	24.9	21.1	24.4
15	24.7	21.9	23.8
16	>40	>40	38.8
17	26.3	22.8	24.9
18	28.7	23.6	27.5
19	33.7	28.6	32.9
20	26.1	22.6	25.9
21	37.5	>40	37.2
22	39.7	>40	38.6
23	29.7	24.3	28.5
24	33.9	35.1	33.6
25	29.8	24.5	29.5
26	36.5	32.9	35.3
27	28.7	23.9	27.9
Pandemic A/H1N1v virus
1	32.9	27.2	31
2	32.2	28.2	30.5
3	35.7	34.8	35.3
4	28.7	22.7	26.9
5	34.7	26.7	27.8
6	29	22.8	34.8
7	36.3	29	32.8
8	34.9	28.3	25
9	27.8	25.1	>40
10	30.3	20.4	32.1
11	33.5	27.3	31.3
12	33.3	27	27.3
13	29	23.7	32.6
14	34.8	28	34
15	35.7	30.3	30
16	31.5	25.1	30.7
17	32.4	26.1	36.7
18	32.5	26.1	32.9
19	34.7	28.5	30.9
20	31.1	25.4	29.3
21	30.8	25.5	31.8
22	33.3	27.5	27.7