Table 3.
Primer sequences used for PCR detection of IHHNV.
| Primer designation | Product | Sequence | Application | Reference |
|---|---|---|---|---|
| 389F | 389 bp | 5′-CGG-AAC-ACA-ACC-CGA-CTT-TA-3′ | All genetic variants | GenBank AF218266 |
| 389R | 5′-GGC-CAA-GAC-CAA-AAT-ACG-AA-3′ | |||
| 77012F | 356 bp | 5′-ATC-GGT-GCA-CTA-CTC-GGA-3′ | All genetic variants | GenBank AF218266 |
| 77353R | 5′-TCG-TAC-TGG-CTG-TTC-ATC-3′ | |||
| 392F | 392 bp | 5′-GGG-CGA-ACC-AGA-ATC-ACT-TA-3′ | All genetic variants | Tang et al. (2000) |
| 392R | 5′-ATC-CGG-AGG-AAT-CTG-ATG-TG-3′ | |||
| 309F | 309 bp | 5′-TCCAACACTTAGTCAAAACCAA -3′ | Infectious virus only | Tang and Lightner (2006) |
| 309R | 5′-TGTCTGCTACGATGATTATCCA-3′ | |||
| 831F | 831 bp | 5′-TTGGGGATGCAGCAATATCT-3′ | IHHNV-realted sequence in P. monodon genome | Tang and Lightner (2006) |
| 831R | 5′-GTCCATCCACTGATCGGACT-3′ | |||