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. 2011 Jan 5;106(1):110–130. doi: 10.1016/j.jip.2010.09.012

Table 5.

Primer set for a single step RT-PCR method for detection of all known genomic variants (strains) of TSV (from Nunan et al. 1998).

Primer Product Sequence G:C ratio Annealing Temperature
9992F 231 bp 5′-AAGTGAACAGCCGCGCTT-3′ 55 69EC
9195F 5′-TCAATGAGAGCTTGGTCC-3′ 50 63EC