Table 1.

Synthetic oligonucleotides used in this study

Name	Primer sequence	Description
γSpeI−119	GCTATTTCTGCACTAGTTTCTTTATG	sgRNAγ promoter large-scale mapping
γSpeI−78	GACTTTAATAAGTACTAGTTGCTGTTTAATTG	sgRNAγ promoter large-scale mapping
γSpeI−38	GAAATTTGTGGATAAACTAGTGAGAAAAGAC	sgRNAγ promoter large-scale mapping
γSpeI−18	GAAAAGACTTCGACTAGTGAACTCTAGG	sgRNAγ promoter large-scale mapping
γSpeI+11	GTTTAAATCTACTAGTTTTACCTTCGC	sgRNAγ promoter large-scale mapping
β1KpnI−107	CCAGATGCCGAGGTACCCGTGACCTGCTG	sgRNAβ1 promoter large-scale mapping
β1KpnI−74	GCGGTAAAAGGGGTACCATATGTATC	sgRNAβ1 promoter large-scale mapping
β1KpnI−34	TATCTATTTTCGGTACCTTTTAGTTTTTG	sgRNAβ1 promoter large-scale mapping
β2+9SmaI	GGCGAGTAACTCCCGGGGTTATTCTGCAA	sgRNAβ2 promoter large-scale mapping
β2KpnI−110	GGAAAGCTTTAGCAGGGTACCATTTGCGTTTAG	sgRNAβ2 promoter large-scale mapping
β2KpnI−76	CTTTGAGCAGACAGGTACCGAAGTTAATCATC	sgRNAβ2 promoter large-scale mapping
β2KpnI−52	GTTAATCATCAGGGCGGTACCGGAAATTCGTC	sgRNAβ2 promoter large-scale mapping
β2KpnI−28	ATTCGTCAAGCATTGGTACCAGGTGATATTGAC	sgRNAβ2 promoter large-scale mapping
5′sgγBamHI	GGATCCGGTGCTTGATGCTTTGGATAAG	PCR amplification of sgRNAγ promoter
3′sgγBamHI	GGATCCCCACAGTAAGTACTTGTAGTTAAG	PCR amplification of sgRNAγ promoter
5′sgβ1BamHI	GGATCCGAGTGCGAGACTCCCAGTG	PCR amplification of sgRNAβ1 promoter
3′sgβ1BamHI	GGATCCCACCTTTCACTGAATCAG	PCR amplification of sgRNAβ1 promoter
5′sgβ2BamHI	GGATCCGTGCCCTGGCAATTGATGTGCAAG	PCR amplification of sgRNAβ2 promoter
3′sgβ2BamHI	GGATCCCGACACCGATTCCGGCGACAATTGG	PCR amplification of sgRNAβ2 promoter
β1134BamHI	GCTCAACCGAGTAGGATCCATGACCTTAAAGG	Engineer BamHI site from nts 1132–1137 in RNAβ
γ2339BamHI	CCAAAAGCATGCGGATCCGTATGATTCAC	Engineer BamHI site from nts 2338–2343 in RNAγ
5′sgβ1−74BamHI	GGATCCCATATGTATCTTATTTATT	PCR amplification of sgRNAβ1 promoter
BSMV3′SpeI	GGGAAGACCACTAGTCATGCAAGC	Change MluI site to SpeI at BSMV RNAγ3′ end

Note. Restriction sites are in bold.