Table 1.
Construction of PRRSV infectious clone.
| Name | Yeara | Genotypeb | Isolate | GenBank # | Cell type for |
Vector | Promoter | Genetic markerd | Reference | |
|---|---|---|---|---|---|---|---|---|---|---|
| Transfection | Passage | |||||||||
| pABV414e | 1998 | I | Ter Huurne (TH) | N/A | BHK-21 | PAM/CL2621 | pOK12 | T7 | N/A | Meulenberg et al. (1998) |
| pABV416e | 1998 | I | Ter Huurne (TH) | N/A | BHK-21 | PAM/CL2621 | pOK12 | T7 | N/A | Meulenberg et al. (1998) |
| pABV437 | 1998 | I | Ter Huurne (TH) | N/A | BHK-21 | PAM/CL2621 | pOK12 | T7 | pacI(3′UTR) | Meulenberg et al. (1998) |
| N/A | 2003 | II | VR-2332 | AY150564 | BHK-21C | PAM/MARC-145 | pOK12 | T7 | BstZ17I (ORF1a)/HpaI(3′UTR) | Nielsen et al. (2003) |
| pFL12 | 2004 | II | NVSL#97-7895 | AY545985 | MARC-145 | PAM/MARC-145 | pBR322 | T7 | BsrGI(ORF1a) | Truong et al. (2004) |
| pT7-P129 | 2005 | II | P129 | AF494042 | MARC-145 | MARC-145 | pCR2.1 | T7 | C1559T/A12622G | Lee et al. (2005) |
| pCMV-S-P129 | 2005 | II | P129 | AF494042 | MARC-145 | MARC-145 | pCMV | hCMV | C1559T/A12622G | Lee et al. (2005) |
| pSD01-08 | 2006 | NA If | SD 01-08 (P34) | DQ489311 | BHK-21 | PAM/MARC-145 | pACYC177 | T7 | ScaI(ORF7) | Fang et al., 2006a, Fang et al., 2006b |
| pPP18 | 2006 | II | Prime Pac (PP) | DQ779791 | MARC-145 | PAM/MARC-145 | pOK12 | T7 | SpeI(ORF1a) | Kwon et al. (2006) |
| pVR-V7 | 2007 | II | VR2332 | DQ217415 | MA-104/MARC-145 | MA-104/MARC-145 | pOK12HDV-PacI | T7 | G7329A/T7554C | Han et al. (2007) |
| pWSK-DCBA | 2007 | II | BJ-4 | EU360128 | MARC-145 | PAM/MARC-145 | pWSK29 | SP6 | VspI(ORF1b) | Ran et al. (2008) |
| pAPRRS | 2008 | II | APRRS | N/A | MA-104 | MA-104 | pBluescript SK(+) | T7 | N/A | Yuan and Wei (2008) |
| pORF5M | 2008 | II | APRRS | N/A | MA-104 | MA-104 | pBluescript SK(+) | T7 | MluI (ORF5) | Yuan and Wei (2008) |
| pJX143 | 2008 | II | JX143 | EF488048 | MA-104 | MA-104 | pBlueScript II SK (+) | T7 | N/A | Lv et al. (2008) |
| pJX143M | 2008 | II | JX143 | N/A | MA-104 | MA-104 | pBlueScript II SK (+) | T7 | MluI (ORF6) | Lv et al. (2008) |
| pWSK-JXwn | 2009 | II | JXwn06 | N/A | BHK-21 | MARC-145 | pWSK29 | SP6 | BstBI(ORF1a) | Zhou et al. (2009) |
| pWSKHB-1/3.9 | 2009 | II | HB-1/3.9 | N/A | BHK-21 | MARC-145 | pWSK29M | SP6 | MluI (ORF1a) SifI (ORF1b) |
Zhou et al. (2009) |
| pHuN4-F112 | 2011 | II | HuN4-F112 | N/A | BHK-21 | MARC-145 | pBlueScript II SK (+) | SP6 | MluI (ORF6) | Zhang et al. (2011) |
| pACYC-VR2385-CA | 2011 | II | VR2385-CA | N/A | BHK-21 | MARC-145 | pACYC177 | T7 | Sph I(ORF1a) | Ni et al. (2011) |
| pIR-VR2385-CA | 2011 | II | VR2385-CA | N/A | BHK-21 | MARC-145 | pIRES-EGFP2 | CMV | Sph I(ORF1a) | Ni et al. (2011) |
| pSHE | 2013 | I | SHE(AMER-VAC-PRRS/A3) | GQ461593 | BHK-21 | MARC-145 | pB-ZJS | CMV | N/A | Gao et al. (2013) |
| pCMV-SD95-21 | 2013 | II | SD95-21 | KC469618 | BHK-21 | MARC-145 | pACYC177 | CMV | N/A | Li et al. (2013) |
N/A, not application.
The individual time of PRRSV infectious clones construction referred to the date of each publication.
Genotype I and II PRRSV represents European and North America strains, respectively.
c Sequences of full-genome cDNA clone rather than complete genome sequences of parental virus are listed.
The genome area in which the restricted enzyme sites are introduced is indicated with brackets. The position where single mutations are introduced is given.
Genome-length cDNA clones of pABV414 and pABV416 encode identical viral protein sequences except for one amino acid at position 1084 in ORF1a, which are a Pro in pABV414 and a Leu in pABV416.
The abbreviation, NA I, in the genotype column represents genotype I PRRSV isolated in North America.