Table 4.

Constructions of chimeric viruses.

Swapped regiona					Substituentb				Viability
Virus	Strain	Infectious clonec	Genome region	Position aa	Virus	Strain	Genome region	Position aa
EAV	Bucyrus	pA45	ORF5	1–114	LDV	P	GP5	1–64	+
					PRRSV	IAF-Klop	GP5	1–64	+
					SHFV	LVR 42–0/M6941	GP7	1–138	−
					SinV	San Juan	E1	1–428	−
					VSV	HR	G	1–402	−
				Whole	EAV	A45-80.4	GP5	Whole	+
						5rUCD	GP5	Whole	+
						5r6D10	GP5	Whole	+
						5rVAC	GP5	Whole	+
						5rKY84	GP5	Whole	+
						5rIL93	GP5	Whole	+
						5rCA95	GP5	Whole	+
						5rWA97	GP5	Whole	+
						5rATCC	GP5	Whole	+
						5r10B4	GP5	Whole	+
			ORF6	17–162	PRRSV	IAF-Klop	M	1–16	−
					LDV	P	M	1–14	−
	ARVAC	prMLVB4/5	ORF5	115–255	PRRSV	IA-1107	ORF5	1–64	+
		prMLVB4/5	ORF5	N/Ad	PRRSV	IA-1107	ORF5	Whole	−
		prMLVB5/6	ORF6	N/Ad	PRRSV	IA-1107	ORF6	Whole	−
		prMLVB4/5/6	ORF6	17–162	PRRSV	IA-1107	ORF6	1–17	+
PRRSV	LV	pABV437	ORF6	1–16	PRRSV	V2332	M	1–16	−
					LDV	P	M	1–14	+
					EAV	Bucyrus	M	1–17	−
		pABV871	ORF6	1–16	PRRSV	V2332	M	1–16	+
					EAV	Bucyrus	M	1–17	+
	VR2332	N/A	ORF2	Whole	PRRSV	JA142	ORF2	Whole	+
			ORF3	Whole	PRRSV	JA142	ORF3	Whole	+
				1–194	PRRSV	JA142	ORF3	1–194	+
				183–255	PRRSV	JA142	ORF3	183–255	+
			ORF4	Whole	PRRSV	JA142	ORF4	Whole	+
			ORF5	Whole	PRRSV	JA142	ORF5	Whole	+
						SDSU73	ORF5	Whole	+
						2M11715	ORF5	Whole	+
						PRRS124	ORF5	Whole	+
			ORF6	Whole	PRRSV	JA142	ORF6	Whole	+
			ORFs5-6	Whole	PRRSV	JA142	ORFs5-6	Whole	+
			ORFs4-6	Whole	PRRSV	JA142	ORFs4-6	Whole	+
			ORFs3-6	Whole	PRRSV	JA142	ORFs3-6	Whole	+
			ORFs2-6	Whole	PRRSV	JA142	ORFs2-6	Whole	+
	APRRS	pAPRRS asc	ORFs2a-4	Whole	PRRSV	SHE	ORFs2a-4	Whole	+
					EAV	vEAV030	ORFs2a-4	Whole	+
			ORFs2a-5	Whole	PRRSV	SHE	ORFs2a-5	Whole	+
			ORF5	Whole	PRRSV	SHE	ORF5	Whole	+
	NVSL# 97–7895	FL12	ORFs2a-7	Whole	PRRSV	PRRSV01	ORFs2a-7	Whole	+

N/A, not application. Whole represents the full sequence of a specific region.

^aThis section provides information of the regions replaced by the counterparts, including the virus, virus strains, the infectious clone used for swapping, and the exact position in which region is changed.

^bThe substituent section indicates the sequences used for substitutions.

^cInfectious clone construct provided here includes both prototypes and modified constructs.