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. 2006 Apr 4;135(2):151–156. doi: 10.1016/j.jviromet.2006.02.012

Fig. 2.

Fig. 2

Results of optimization for RT-LAMP condition. (A) Varied temperature: lane 1, molecular size marker (φ/X174/Hinc II digest); lanes 2–4, RT-LAMP carried out at 60, 63 and 65 °C, respectively. (B) Varied reaction time: lane 1, molecular marker (φ/X174/Hinc II digest); lanes 2–5, LAMP carried out for 15, 30, 45 and 60 min, respectively. All products were electrophoresed on a 2% agarose gel and stained with ethidium bromide.