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Sensitivity of RNA detection by RT-LAMP and nested RT-PCR: (A) RT-LAMP products and (B) nested RT-PCR products. Lane 1, molecular size marker (φ/X174/Hinc II digest); lanes 2–8, RT-LAMP and nested RT-PCR carried out using concentrations of RNA (10−1, 10−2, 10−3, 10−4, 10−5, 10−6 and 10−7), respectively. All products were electrophoresed on a 2% agarose gel and stained with ethidium bromide.
