Fig. 1.

Schematic representation of hepatitis E virus ORF1: HEV ORF1 region encodes nonstructural polyprotein that contains methyltransferase (MeT), Y, papain-like cysteine protease (PCP), variable (V), X (macro), RNA helicase (Hel), and RNA-dependent RNA polymerase (RdRp) domains. The conserved motifs, typical of the helicase superfamily I (designated as I–VI) are shown in the enlarged view of HEV helicase (960–1204 aa). The amino acid changes done in the helicase region of pET15b.HEV Hel expression clone and HEV Rluc replicon are indicated in ‘Bold’ text.