Table 2.

Comparative NTPase and unwinding activities of helicase mutants.

Helicase protein	% NTPase activity	% unwinding activity
L1110F Hel	80.3 ± 4.3	112.2 ± 10.2
V1120I Hel	70.4 ± 4.4*	103.3 ± 9.7
L1110F/V1120I Hel	98.2 ± 2.3	110.6 ± 7.5
Walker A	25.1 ± 3.9*	18.6 ± 7.3*
Walker B	4.43 ± 6.9*	0 ± 0*
WILD	100 ± 3.3	100 ± 8.3

Comparative activities were calculated by assuming the activity of wild type helicase as 100%. ±: standard error of the mean.

^*p ≤ 0.05: significant difference as compared to wild type protein.