Table 2.

Matching and mismatching influenza A, B and C synthetic oligonucleotides relative to the TaqMan^® probes.

Notes: In an assessment of 2757 Influenza A NP sequences from GenBank (2005), the frequency of variant (mismatching) nucleotides relative to the Influenza A TaqMan^® probe was, at pos 1: 1183 T, pos 2: 183 T and pos 3: 1240 A. The mismatch positions in question are underlined, together with the mismatch position number. In an assessment of 95 Influenza B sequences, the frequency of variant (mismatching) nucleotides was, at pos 1: 15 A, pos 2: 15 A, pos 3: 6 A and pos 4: 4 A. There were no appreciable mismatches (counting >1 mismatch in the entire set of 88 sequences) in the set of Influenza C sequences. The oligonucleotides were purchased from Thermo Hybaid. Positions with occasional mismatches relative to probes are indicated in pink color, the primer positions are indicated in blue and the probe positions in yellow.