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. 2009 Oct 29;163(2):313–322. doi: 10.1016/j.jviromet.2009.10.017

Table 3.

Matching and mismatching influenza A synthetic oligonucleotides relative to the MegaBeacon probe.

3.2.

Notes: A new Influenza A multiple alignment was generated: in an assessment of 2500 Influenza A NP sequences from GenBank (2008), the 10 displayed variant positions were found, Thus, the MegB target region (yellow shade) contained up to 10 mismatching nucleotides (magenta shade) versus the MegB probe. Primer targets are shaded cyan. Oligonucleotides of all 10 variants were purchased from Thermo Hybaid to test the mismatch tolerance of the MegB.