Table 2.
Conserved primers for whole genome amplification and sequencing of WU/KI polyomaviruses
| Oligo name | Sequence (5′ → 3′) | Positiona | Product (bp) |
|---|---|---|---|
| WU/KI_4969F | TTGCAGTTTTAAATATAAAGAATTAAG | 4969–4995 | 1395 |
| WU/KI_761R | ACAGTAGYAGCTGCTTCAGATAT | 761–739 | |
| WU/KI_1134R | GGCAATTCTATTGAACACGTCCT | 1134–1112 | |
| WU/KI_989F | CCCCCTACYCAGGAATGGCA | 989–1008 | 1560 |
| WU/KI_1990R | ATTWGGWATATCAGGGGGAGCAA | 1990–1968 | |
| WU/KI_2548R | CCTTCTTTGTCTAAARTGTAGCCTA | 2548–2524 | |
| WU/KI_2407F | ACTRTTGGATGAAAATGGCATTGG | 2407–2430 | 1695 |
| WU/KI_2870F | GCAAAYTCAGTAAGGCCTATATA | 2870–2892 | |
| WU/KI_4101R | CATAGAGTTAGTGCTRTTAATAACTT | 4101–4076 | |
| WU/KI_3780F | TACTAYTGCATTTTTCACACTCTTC | 3780–3804 | 1389 |
| WU/KI_4703R | TGTGGATAGAGTGYTACTGCTA | 4703–4682 | |
| WU/KI_5168R | AAGAAGGCAAAATGGATAAAACTTT | 5168–5144 |
a
Position based on nucleotide sequence of WU polyomavirus (NC_009539).