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. 2010 Nov 24;149(3):316–323. doi: 10.1016/j.vetmic.2010.11.026

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Crown copyright © 2010 Published by Elsevier B.V. All rights reserved.

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Fig. 1 — Phylogenetic relationship of Canadian porcine astroviruses (boxed) and prototypical astrovirus species. (a) Tree based on partial RdRp coding region (∼300 nt) amplified using pan-astrovirus primers. (b) Tree based on complete ORF2 coding region (∼800 aa). Phylogenetic trees were generated using the neighbour-joining method implemented in the program MEGA4. Bootstrap values are expressed in percentage based on 1000 replications and indicated at the node. Bootstrap values of ≤70% were hidden. Scale bar represents distance expressed as nucleotide or amino acid substitutions per site. Accession numbers for the AstV strains characterized in this study are from HM756258 to HM756273.