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. 2007 Nov 6;32(6):613–626. doi: 10.1016/j.dci.2007.10.002

Figure 4.

Figure 4

Photomicrographs of semi-thin sections of lymphoid organ (LO) tissue stained with toluidine blue. (a) Control P. vannamei at d0 showing normal LO tubules (LOT) and absence of LO spheroids (LOS). (b) LO tissue of a moribund shrimp from the YHV source shrimp (also YHV-positive control shrimp) at d2 after YHV injection showing numerous pyknotic nuclei (arrows), karyorrhectic nuclei and cytoplasmic inclusions (arrow heads). (c) LO tissue of moribund shrimp from test Group 1 (immersion challenge) at d5 after YHV challenge showing features similar to those of the YHV-injected shrimp in (b). (d) Surviving shrimp at d14 showing the presence of LOS, some with clearly visible capsular sheaths (*), pyknotic nuclei (arrows) and vacuolated cells.