Figure 5.

Confocal laser scanning micrographs (CLSM) showing immunofluorescent labeling for gp116 and p20 of YHV in LO sections of P. vannamei challenged with YHV by the water-borne route (a and d). Tissue from moribund shrimp collected at d5 showing an intense, positive yellow-green immunofluorescent signal for gp116 (a) and p20 (d) in LOT matrices and in clusters of aggregated hemocytes (asterisks *) in the matching phase contrast images (c and f). Photomicrographs (b) and (d) show combined images that include nuclear staining (orange). (g–j) Photomicrograps of tissue from surviving shrimp collected at d14 exhibiting weak immunopositive reactions for gp116 (g) and p20 (i) in LOS and no reactions in LOT. Photomicrographs (g) and (i) show respective combined images that include nuclear staining.