Table 3.
Real time PCR primers for determination of PI3V types.
| Primer | Sequence |
|---|---|
| PI3V U Forwarda | AGA GCA CTC RAT TTA CAG ARA GGb |
| PI3V U Reverse | GTA TCY GCA TTG TTN AGG ACA TTc |
| PI3V U Probe | AGA CAA GAG AGT TGT RTG TGT GG |
| PI3V B Forward | TTG TGT GTG TGG CAA ATG TC |
| PI3V B Reverse | TTC TGT GTG ATT CGT CCA TTT |
| PI3V B Probe | ACT CCG ATC ATC AGG CCC GC |
| PI3V C Forward | AAT AGG TGA CCC ACC CAA AC |
| PI3V C Reverse | CAG ATC ATC GAC ACT CCC ATA TC |
| PI3V Probe | TTG AGA TGG AGC GGT CAA AGG |
a
U- Universal primer for detection of all PI3V isolates.
b
R-purine.
c
N-any base.