Fig. 3.

Virus infectivity after acid treatment. 293 cells were infected with Ad2 or Ad41 using two different MOI (see Materials and methods). Virus portions were untreated or exposed to HCl at pH 2 or 4 for 1 min at RT or at 37 °C. Exposure was stopped by dilution in culture medium. Virus progeny was estimated by fluorescence as described in Materials and methods. The results in percent show the activation or inhibition of infection upon HCl treatment (above or below zero line, respectively), where baseline is the infection level for the untreated virus.