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. 2004 Mar 16;322(1):93–104. doi: 10.1016/j.virol.2004.01.020

Fig. 4.

Fig. 4

Fiber interaction with CAR. Native purified proteins (0.5 μg) were dotted onto the nitrocellulose membrane before or after acid treatment for 1 min at pH 2.0 then allowed to interact with 1 μg of purified recombinant extracellular domain of human CAR. CAR binding was visualized by Western blot with anti-CAR antibody. Ad41 short and long pentons, Ad5 fiber, and Ad3 penton-dodecahedron (composed of 12 pentons, complexes of base and fiber) are noted as SP41, LP41, F5, and P3, respectively. The positive (CAR) and negative (PBS) controls of anti-CAR antibody are shown on the right.