Fig. 7.
Comparison of glycosphingolipid-binding properties of Ad41 with Ad2. The nitrocellulose membrane containing 100 pmol of the indicated sphingolipids was incubated with the purified viruses as described in Materials and methods. The ligands bound to the membrane-immobilized lipids were detected with specific antibody. Binding was performed at pH 6.0 without Mg2+ (*) or at pH 7.5 in presence of Mg2+. Lipid positions are indicated in the diagram: 1, sphingosine; 2, sphingosine 1-phosphate; 3, phytosphingosine; 4, ceramide; 5, sphingomyeline; 6, sphingosyl-phosphocholine; 7, lysophosphatidic acid; 8, myriocin; 9, monosialoganglioside GM1; 10, disialoganglioside GD3; 11, sulfatide; 12, sphingosylgalactoside (psychosine); 13, cholesterol; 14, phosphatidylcholine; 15, lysophosphophatidylcholine; 16, blank.