Table 1.
Sequences and details of the primers and probe used for SFTSV RT-RPA assays, RT-qPCR assays and RT-PCR assays.
| Assay | Primer/Probe | Sequence (5′-3′) | Number of base pairs |
|---|---|---|---|
| RT-RPA | RPA-L-F4a | ATCACAATCCAGCTCTCTGAAGCGTATAAG | 128 |
| RPA-L-R4b | CATGTTGGACAGAACTCCTCCTGACGACACTAC | ||
| RPA-L-P2c | AGGCAGCATACAGGACAAAGATAGAAAAG[dT-FAM] [dSpacer][dT-BHQ1]AGGGACCCAATCTCAA-[C3 Spacer] | ||
| RT-qPCR | qPCR-Fa | CTTCTTTGGGGTTATTGTAGTGT | 131 |
| qPCR-Rb | CCATAGACCTAGCCTTAGTGT | ||
| RT-PCR | st-Fa | TGCCAGACCTAGATGTGACTG | 569 |
| st-Rb | CTCAAGCTCTTCTTCGCTCT |
a
F indicates forward primer.
b
R indicates reverse primer.
c
P indicates exo-probe. For probe modifications: dT-FAM, thymidine nucleotide carrying FAM fluorophore; dSpacer, a tetrahydrofuran residue; dT-BHQ1, thymidine nucleotide carrying BHQ-1 quencher; C3 Spacer, 3′-block.