Table 1.

Oligonucleotides used for amplification of complete HEV coding genome initial reaction (1st R) and nested reaction (2nd R). Tm: annealing temperature. I: inosine.

1st R	B1	B2	B3	B4	B5	B6	B7	B8	B9
Name	E3_12S	E3_561S	E3_22S	E3_1527S	E3_2032S	E3_3475S	E3_4759S	E3_5236S	E3_6474S
Sequence (5′-3′)	ACGYATGTGGTCGAWGCCATG	ATGGCCCGICAYGGGATGAC	TCGAWGCCATGGAGGCCCA	GGCYTAYGARGGITCIGARGT	TRTGGYTRCAYCCYGAGGG	CTYGGYGAYCCIAAYCAGAT	ATGGARGARTGYGGYATGCC	TGCCTATGYTG CCCGCGC	GCIGCYACRCGI TTYATGAA
Name	E3_987A	E3_2091A	E3_1584A	E3_3025A	E3_3781A	E3_5236A	E3_5597A	E3_6648A	E3_7371A
Sequence (5′-3′)	AARAGCATRAGCCGRTCCCA	AAIGGRTTIGCIGAYTCCCA	CCRTGRACIGCRTARGTCCC	GGGCGRTGRTTRCGYTCCCA	CTRGCRTCRGCYGTRGCTAT	ACATCRACACARACCTGCGC	GGGTTGGTTGGATGAATATAGGGG	ACWGYYGGCT CACCATTGGC	TTYTAAGRCGC TGAAGYTCAG
Size (nt)	975	1530	1562	1498	1749	1761	838	1412	897
Tm (°C)	60	60	60	57	57	50	57	57	57

2nd R	B1n	B2n	B3n	B4n_1	B4n_2	B5n_1	B5n_2	B6n_1	B6n_2	B7n	B8n	B9n
Name	E3_22S	E3_984S	E3_120S	E3_1527S	E3_2032S	E3_2896S	E3_3316S	E3_3592S	E3_4192S	E3_4895S	E3_5478S	E3_6587S
Sequence (5′-3′)	TCGAWGCCATGGAGGCCCA	ATYTGGGAYCGGCTYATGCT	GCTGTGGTGGTYCGGCCGTT	GGCYTAYGARGGITCIGARGT	TRTGGYTRCAYCCYGAGGG	AGGRTYGARCAGAAYCCIAAGAG	GAGCTYCGIAAYAGYTGGCG	GAYGTITGYGARCTYATACG	ATAGTYCAYTGYCGIATGGC	GGAAYACYGTY TGGAAYATGGC	TGCCTATGYTG CCCGCGC	CCGACAGAATT RATTTCGTCGGC
Name	E3_561A	E3_1584A	E3_1314A	E3_2091A	E3_2923A	E3_3563A	E3_3781A	E3_4384A	E3_5227A	E3_5592A	E3_6631A	E3_7302A
Sequence (5′-3′)	GTCATCCCRTGICGRGCCAT	CCRTGRACIGCRTARGTCCC	CGRCAYTGIGCRTARAACTG	AAIGGRTTIGCIGAYTCCCA	GTYTCICGRTAYGCYGCCTC	CGRTGIGTIACRTGCCACCA	CTRGCRTCRGCYGTRGCTAT	TCCTGRCCCTTYTCIACCAT	CARACCTGCGCIACRTTCGT	TTGGTTGGATG AATATAGGGGA GGG	CCCGCGC GGCYGARACRA CIGGGCGGG	TCMGGRCARAA ATCATCRAAAGT
Size (nt)	539	600	1194	564	891	667	465	792	1035	697	1076	715
Tm (°C)	60	60	57	54		54		50		57	57	57