Antibodies |
|
CD14-FITC (RMO52 clone) |
Beckman Coulter |
Cat#IM0645U; RRID: AB_130992
|
CD45-PC5 (J33 clone) |
Beckman Coulter |
Cat#IM2653U; RRID: AB_10641226
|
HLA-DR-RD1 (9-49 clone) |
Beckman Coulter |
Cat#6604366; RRID: AB_2832962
|
IgG1-PE isotype control (679.1Mc7 clone) |
Beckman Coulter |
Cat#A07796; RRID: AB_2832963
|
IgG1-FITC isotype control (679.1Mc7 clone) |
Beckman Coulter |
Cat#A07795; RRID: AB_2832964
|
CD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) |
Beckman Coulter |
Cat#A07733; RRID: AB_2832965
|
CD3-FITC/CD8-PE Antibody Cocktail |
Beckman Coulter |
Cat#A07734; RRID: AB_2832966
|
CD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) |
Beckman Coulter |
Cat#A07735; RRID: AB_1575965
|
CD19-FITC/CD5-PE Antibody Cocktail |
Beckman Coulter |
Cat#IM1346U; RRID: AB_2832967
|
Quantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) |
BD Biosciences |
Cat#340827; RRID: AB_400137
|
Mouse Anti-Human IL-6-PE (AS12 clone) |
BD Biosciences |
Cat#340527; RRID: AB_400442
|
|
Chemicals, Peptides, and Recombinant Proteins |
|
VersaLyse Lysing Solution |
Beckman Coulter |
A09777 |
Fixative Solution |
Beckman Coulter |
A07800 |
eBioscience™ Brefeldin A Solution (1000x) |
Invitrogen |
00-4506-51 |
IntraPrep Permeabilization Reagent |
Beckman Coulter |
A07802 |
RPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES |
Biowest |
L0496 |
Lymphosep, Lymphocyte Separation Media |
Biowest |
L0560 |
PBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium |
Biowest |
L0615 |
FBS Superior; standardized Fetal Bovine Serum, EU-approved |
Biochrom |
S0615 |
Gentamycin Sulfate BioChemica |
PanReac AppliChem |
A1492 |
Penicillin G Potassium Salt BioChemica |
PanReac AppliChem |
A1837 |
Lipopolysaccharides from Escherichia coli O55:B5 |
Sigma-Aldrich |
L2880 |
|
Critical Commercial Assays |
|
Human IL-1β uncoated ELISA |
Invitrogen |
88-7261 |
Human TNF-α uncoated ELISA |
Invitrogen |
88-7346 |
Human IL-6 ELISA |
Invitrogen |
88-7066 |
Human IL-17A (homodimer) uncoated ELISA |
Invitrogen |
88-7176 |
Human Ferritin ELISA |
ORGENTEC Diagnostika GmbH |
ORG 5FE |
Human IFN-γ ELISA |
Diaclone |
950.000.192 |
|
Software and Algorithms |
|
GraphPad Prism |
Graphpad Software |
https://www.graphpad.com |
SPSS |
IBM |
https://www.ibm.com/analytics/spss-statistics-software |