Table 2.

Nucleotide length, melting temperature (T_m) and GC content of the probes and primers used in quantitative real-time RT-PCR

Specificity		Start (nt)	End (nt)	Length	Tm (°C)	%GC
CymMV RdRp	TaqMan probe	1215	1238	24	69	58
	Forward primer	1191	1213	23	59	43
	Reverse primer	1265	1287	23	59	43
CymMV CP	TaqMan probe	5600	5625	26	69	50
	Forward primer	5580	5597	18	60	61
	Reverse primer	5649	5668	20	58	50
ORSV RdRp	TaqMan probe	120	149	30	68	43
	Forward primer	100	118	19	58	58
	Reverse primer	173	195	23	58	43
ORSV CP	TaqMan probe	5817	5841	25	70	56
	Forward primer	5793	5815	23	58	43
	Reverse primer	5866	5882	17	58	65