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. 2008 Nov 5;383(1):22–31. doi: 10.1016/j.virol.2008.09.013

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Copyright © 2008 Elsevier Inc. All rights reserved.

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Fig. 3 — The mutant viruses retained similar virological properties of parental virus. (A). Viral plaque morphology. Marc-145 cells in 6-well plate were infected with passage 5 viruses at an M.O.I of 0.1, or mock-infected with DMEM. The monolayer of infected cells was overlaid with agarose, and stained with crystal violet at 5 dpi. (B). Viral growth kinetics growth analysis. The infection was done as for viral plaque assay, without agarose overlay. The cell supernatants were harvested at the indicated time points, followed by viral titration by plaque assay on Marc-145. Based on viral titer and timepoints, the growth curves were determined: vAPRRS (□), vORF7Ua (), vORF673 (+), vORF56c (▽), v5ND7 (△), vORF12 (○).

Inline graphic — The mutant viruses retained similar virological properties of parental virus. (A). Viral plaque morphology. Marc-145 cells in 6-well plate were infected with passage 5 viruses at an M.O.I of 0.1, or mock-infected with DMEM. The monolayer of infected cells was overlaid with agarose, and stained with crystal violet at 5 dpi. (B). Viral growth kinetics growth analysis. The infection was done as for viral plaque assay, without agarose overlay. The cell supernatants were harvested at the indicated time points, followed by viral titration by plaque assay on Marc-145. Based on viral titer and timepoints, the growth curves were determined: vAPRRS (□), vORF7Ua (), vORF673 (+), vORF56c (▽), v5ND7 (△), vORF12 (○).