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. 2020 Apr 14;11:661. doi: 10.3389/fimmu.2020.00661

FIGURE 1.

FIGURE 1

Shift in T lymphocyte populations. Populations of (A) Th1 (IFNγ+CD4), (B) IFNγ-producing CD8+ lymphocytes, (C) Th17 (IL-17+CD4), (D) T regulatory lymphocytes (Tregs) (CD4+FoxP3+CD25hiCD127-) with (E) CD25 expression on their surface were analyzed by flow cytometry in cultures from 20 T1D patients (55% female) and 12 healthy donors (50% female) after 7 day cultivation with or without autologous NET fragments. (F) Induction of Th1, Th17, Treg, and IFNγ+CD8 T cells isolated CD3+ lymphocytes co-cultured with or without autologous NETs for 7 days in T1D patients (n = 7) evaluated by flow cytometry. Statistical analysis was performed using a two-tailed Wilcoxon paired t-test and an unpaired t-test. Values of p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***) were considered statistically significant.