Fig. 4.

Cysteine 48 of PRRSV-Gp5 forms a disulphide bond with the M protein. Full-length genes of Gp5 from PRRSV VR-2332 (fused via the linker GS to a 7×His-tag) and M (fused via the same linker to the HA-tag) were co-expressed with baculovirus in insect cells as described (Thaa et al., 2013). Cells were lysed with reducing (+DTT) or non-reducing (−DTT) SDS-PAGE buffer, followed by SDS-PAGE (equal amounts were applied) and Western blot with anti-HA and anti-His antibodies, respectively. The numbers left to each blot indicate the mobility of molecular weight markers in kDa. Mock indicates that cells were infected with non-recombinant Baculovirus. (A) Western blot of cells expressing Gp5-His wt and M-HA. (B) Western blot of cells expressing Gp5-His C48S with M-HA.