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. 2011 Feb 22;173(2):259–265. doi: 10.1016/j.jviromet.2011.02.014

Fig. 1.

Fig. 1

(a) Dynamic range of the developed RT-PCR assay. Amplification curves (Delta Rn vs. Cycle number) and standard curve (Ct vs. log10 concentration) for serial dilutions of the plasmid with C823 nucleotide. Assay dynamic range was linear between template concentrations ranging from 4.76 × 101 copies/reaction to 4.76 × 108 copies/reactions. (b) Dynamic range of the developed RT-PCR assay. Amplification curves (Delta Rn vs. Cycle number) and standard curve (Ct vs. log10 concentration) for serial dilutions of the plasmid with C823T nucleotide. Assay dynamic range was linear between template concentrations ranging from 5.29 × 102 copies/reaction to 5.29 × 109 copies/reactions.