Table 1.
Primer and probe sequences designed for detection of the C823T mutation. The SNP position is highlighted in bold and underlined.
| Assay | Oligonucleotide name | Oligonucleotide sequence (5′–3′) | Amplicon (bp) | Locationa | Source |
|---|---|---|---|---|---|
| Sequencing (Partial NA) | NA675F_nested_sw | AACACAAGAGTCTGAATGTGC | 415 | 675–695 | In-house |
| NA1089R_nested_sw | ACCGTTTCTTGAACTAATGCTT | 1089–1110 | |||
| Real-time RT-PCR | SOIV_NA_781F | GGAAAGATAGTCAAATCAGTCGAAATG | 781–807 | In-house | |
| SOIV_NA_878R | CTGCACACACATGTGATTTCACTAG | 98 | 854–878 | ||
| SOIV_Osel_SEN | FAM – CCTCATAGTGATAATTA – MGB | 816–832 | |||
| SOIV_Osel_RES | VIC – CCTCATAGTAATAATTAG – MGB | 815–832 | |||
a
Nucleotide numbering is based on the neuraminidase (NA) gene of influenza A virus (A/California/04/2009(H1N1), GenBank accession no. GQ117044).