Table 2b.
Performance of RT-PCR assay using SOIV_Osel_RES probe in comparison to the gold standard Sanger sequencing method.
| Sequencing result for pandemic (H1N1) 2009 virus | Samples (N) | RT-PCR assay |
Sensitivity (percentage) | Specificity (percentage) | |
|---|---|---|---|---|---|
| + | − | ||||
| Sensitive (C823) | 25 | 0 | 25 | 0.0 | 100.0 |
| Resistant (C823T) | 15a | 15 | 0 | 100.0 | 100.0 |
| Resistant admixtures (C823/C823T) | 9 | 7 | 2b | 77.8 | 100.0 |
| Negative for IFVA | 20 | 0 | 0 | 0.0 | 100.0 |
a
Fifteen samples contained 100% T at nucleotide position 823.
b
Two admixtures had a low proportion of T at position 823 and were not detected by the SOIV_Osel_RES probe.