Table 3b.
Detection of mixed templates prepared using quantitated in vitro transcribed RNA at a low copy number.
| % of template 823C RNA | % of template 823T RNA | Copies/reaction of total RNAa | Results for resistant probe | Results for sensitive probe |
|---|---|---|---|---|
| 100 | 0 | 4.76E+02 | Not detected | Detected |
| 95 | 5 | 4.78E+02 | Not detected | Detected |
| 80 | 20 | 4.87E+02 | Detected | Detected |
| 60 | 40 | 4.98E+02 | Detected | Detected |
| 50 | 50 | 5.03E+02 | Detected | Detected |
| 40 | 60 | 5.07E+02 | Detected | Detected |
| 20 | 80 | 5.18E+02 | Detected | Detected |
| 5 | 95 | 5.27E+02 | Detected | Not detected |
| 0 | 100 | 5.29E+02 | Detected | Not detected |
a
Total copy number for both variants of in vitro RNA present in the reaction. Copy number for individuals alleles of in vitro RNA used to make the mixtures is indicated in Section 2.