Table 1.

Oligonucleotide primers used for detection of Mycoplasma spp., M. arginini, virulence genes, and fluoroquinolone resistance (QRDR) genes.

Species	Sequence	Target	References
16SrRNA. For Mycoplasma	5⁄- AGA CTC CTA CGG GAG GCA GCA -3⁄ 5⁄- ACT AGC GAT TCC GAC TTC ATG -3⁄	1000bp	Alberto et al. (2006)
Mycoplasma group- specific	5⁄-GGGAGCAAACAGGATAGATACCCT-3/ 5/-TGCACCATCTGTCACTCTGTTAACCTC-3/	280bp	VanKuppeved et al. (1994)
M. arginini	5⁄- TGATCATTAGTCGGTGGAGAGTTC-3⁄ 5⁄- TATCTCTAGAGTCCTCGACATGACTC-3⁄	326bp	Vahid et al. (2009)
Virulence genes
uvrC	5′-TTACGCAAGAGAATGCTTCA-3′ 5′-TAGGAAAGCACCCTATTGAT-3	1626 bp	Perez-Casal et al. (2007)
gapA	5′-ATAGGAGGATCCAAAAGAGTCGCTATCAATGGTTTTGGACG-3′, 5′-GGAAATGGTACCTTACTTAGTTAGTTTAGCAAAGTATGTTAATG-3′	1007 bp
vsp	5/-CTTGGATCAGTGGCTTCATTAGC-3/ 5/-GTCATCATGCGGAATTCTTGGGT-3/	400bp	Alberto et al. (2006)
Fluoroquinolone resistance genes
gyrA	5′-GACGAATCATCTAGCGAG-3′ 5′-GCCTTCTAGCATCAAAGTAGC-3′	531bp	Lysnyansky et al. (2009)__
parC	5′-GAGCAACAGTTAAACGATTTG-3′ 5′-GGCATAACAACTGGCTCTT-3′.	488bp